This course summarizes knowledge in genomics, proteomics, transcriptomics, metabolomics and relative molecular technologies. Topics include an overview of technologies in functional genomics (DNA chip arrays); whole genome expression analysis (EST, MPSS, SAGE, arrays); proteome analysis technology (2D-electrophoresis, protein in situ digestion for mass spectrometric analysis, yeast 2-hybrid analysis. 2-D PAGE, MALDI-TOF spectroscopy); the principles of Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry technologies for metabolomics, including general principles, the strengths and weaknesses of each technique, the requirements for sample preparation and the options for the management of output data. This course explains how to exploit different -ome database resources for investigations via special practical tasks to lectures. Special attention is focused on nutrigenomics, a multidisciplinary science that uses genomics, transcriptomics and proteomics to study metabolic health. This relatively new area of metabolomics has the potential to contribute significantly to advances in nutrition and health.
Prerequisites: Bioinformatics II, Bioinformatics III